Technical Data

030062
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B140 (8B6)
Grade
Ascites
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
969
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD69
CLEC2C; CD69

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, andplatelets Subcellular location: Membrane, Single-pass type II membrane protein Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhanscells and platelets Sequence similarities: Contains 1 C-type lectin domain.

Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment corresponding to human CD69 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Recognizes human CD69.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. EMBO J. 1997 Feb 17;16(4):673-84. 2. Cell Immunol. 2002 Nov;220(1):20-9. 3. Arch Biochem Biophys. 2005 Jun 1;438(1):11-20.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net