Technical Data

030079
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B157
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu Mo
Gene ID
1017
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CDK2 (Cyclin-dependent kinase 2)
p33

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Applications
Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human CDK2 expressed in E. coli.
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
Purity
Purified
Specificity
Human, Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Eur Arch Otorhinolaryngol. 2009 Oct;266(10):1501-7. |Cell Cycle. 2009 Jun 15;8(12):1952-63.|
USBio References
No references available
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