Technical Data

030088
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B166
Grade
Purified
Applications
E FC WB
Crossreactivity
Hu
Gene ID
1050
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CEBPA (CCAAT/enhancer-binding protein alpha)
CEBP; C/EBP-alpha

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Applications
Western Bloting: 1:500-1:2000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human CEBPA expressed in E. coli.
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
Purity
Purified
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Haematologica. 2009 Aug;94(8):1075-84.|Br J Cancer. 2009 Aug 18;101(4):738-40. |
USBio References
No references available
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