Technical Data

030228
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B306
Grade
Ascites
Applications
E IHC WB
Crossreactivity
Hu Mo
Gene ID
2268
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-FGR (Tyrosine-protein kinase Fgr)
SRC2; c-fgr; c-src2; p55c-fgr; p58c-fgr

FGR: Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, also known as SRC2; c-fgr; c-src2; FLJ43153; MGC75096; p55c-fgr; p58c-fgr. It is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Applications
Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human FGR expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Human,Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Immunity. 2005 Feb;22(2):235-46.|2. Biochemistry. 2007 Oct 2;46(39):11023-32.
USBio References
No references available
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