Technical Data

030237
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B315
Grade
Ascites
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
2353
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-FOS
p55; AP-1; C-FOS

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Applications
Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human FOS expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Mol Cell Biochem. 2010 Apr;337(1-2):53-63.|Cancer Invest. 2009 Oct;27(8):816-24.
USBio References
No references available
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