Technical Data

030287
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B365
Grade
Ascites
Applications
E WB
Crossreactivity
Hu
Gene ID
9421
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-HAND1 (Heart-and neural crest derivatives-expressed protein 1)
Hxt; eHand; Thing1; bHLHa27

HAND1: heart and neural crest derivatives expressed 1. The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.

Applications
Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of HAND1 (aa90-190) expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. J Biol Chem. 2002 Apr 12;277(15):12604-12. |2. Mol Cell. 2003 Nov;12(5):1225-37.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net