Technical Data

030326
Clone Type
Monoclonal
Host
Mouse
Source
Drosophila
Isotype
IgG1
Clone Number
13B404
Grade
Ascites
Applications
E WB
Gene ID
33972
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-IHOG (Interference hedgehog)  (Drosophila)
CG9211; CT26314; Dmel\CG9211; ihog; Ihog

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

Applications
Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human IHOG expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
drosophila

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Cell. 2006 Apr 21;125(2):343-57.|2. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17208-13.
USBio References
No references available
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