Mouse Anti-MAP2K4 (Mitogen-activated Protein Kinase Kinase 4)
JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; MAP2K4
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Tissue specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues .
Applications
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human MAP2K4 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Specificity
Recognizes human MAP2K4
