Technical Data

030431
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B509
Grade
Ascites
Applications
E IF WB
Crossreactivity
Hu
Gene ID
4143
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-MATN1 (methionine adenosyltransferase I, alpha, Methionine adenosyltransferase 1, SAMS1)
MAT; SAMS; MATA1; SAMS1; MAT1A

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.

Applications
Western Bloting: 1:500-1:2000. Immunofluorescence: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human MATN1 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Biochem J. 1993 Jul 15;293 ( Pt 2):481-6.|2. Am J Hum Genet. 1997 Mar;60(3):540-6.|3. Am J Hum Genet. 2000 Feb;66(2):347-55.
USBio References
No references available
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