Mouse Anti-Podocalyxin (Podocalyxin-like protein-1, PC, PCLP, PCLP1, PODXL, MGC138240, GCTM-2 Antigen Gp200)
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin): 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human PODXL expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Specificity
Recognizes human PODXL.
