Technical Data

030593
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B671
Grade
Purified
Applications
E FC WB
Crossreactivity
Hu
Gene ID
5906
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-RAP1A
RAP1; KREV1; KREV-1; SMGP21

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Applications
Western Bloting: 1:500-1:2000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human RAP1A expressed in E. coli.
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
Purity
Purified
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Mol Biol Cell. 2009 Apr;20(7):1916-25. |J Cell Sci. 2009 Aug 15;122(Pt 16):2996-3004.|
USBio References
No references available
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