Technical Data

030617
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B695 (2H2C4)
Grade
Supernatant
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SARS, M

SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The complete sequence of the SARS virus release the coronavirus contains 25 open reading frames. SARS-m is a membrane (M) protein which plays a the key player in virion assembly. One of its functions is to mediate the incorporation of the spikes into the viral envelope.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1;10,000 Western Blot: 1;500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of SARS-m protein expressed in E. coli
Form
Supplied as a liquid
Purity
Supernatant
Specificity
Recognizes human coronavirus

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fournel S, Cell Immunol 1993 Aug; 150(1):194-204. 2. J. Virol., Sep 1999; 73: 7441 - 7452.
USBio References
No references available
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