Mouse Anti-SERPINE1 (Plasminogen activator inhibitor 1, Serpin E1)
PAI; PAI1; PAI-1; PLANH1
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000 Western Blot: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant protein fragment corresponding to human SERPINE1 expressed in E. coli.
Form
Supplied as a liquid in PBS,0.5% protein stabilizer, 0.05% sodium azide.
Specificity
Recognizes human SERPINE1.
