Technical Data

030632
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B710
Grade
Ascites
Applications
E FC IHC WB
Crossreactivity
Hu Rt
Gene ID
4090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SMAD5
Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunocytochemistry, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 Immunocytochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human SMAD5 expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Recognizes human SMAD5. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3927-32.|2. Nat Cell Biol. 2008 May;10(5):567-74.
USBio References
No references available
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