Technical Data

030649
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13B727
Grade
Ascites
Applications
E FC WB
Crossreactivity
Hu
Gene ID
6688
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SPI1
OF; PU.1; SFPI1; SPI-1; SPI-A

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications
Western Blot: 1:500-1:2000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human SPI1 (AA: 124-271) expressed in E. coli.
Form
Supplied as a liquid, 0.03% sodium azide.
Purity
Ascites
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.J Biol Chem. 2011 Jan 21;286(3):1675-82.|2.Leuk Res. 2010 Dec;34(12):1636-46.|
USBio References
No references available
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