Technical Data

030742
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1, IgG,k
Clone Number
137CT26.1.1
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo
Gene ID
60
Gene #
ACTB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Actin, beta (ACTB, Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed)
ACTB, Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:200~4000 Immunohistochemistry 1:50~100 Immunofluorescence 1:100~500
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
ACTB Mab is generated from mouse immunized with ACTB recombinant protein.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.
USBio References
No references available
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