030809
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1,kClone Number
282CT3.7.6Grade
AscitesApplications
E WBCrossreactivity
HuAccession #
NP_005171.4Gene ID
648Gene #
BMI1Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-BMI1 (BMI1, PCGF4, RNF51, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51)
BMI1, PCGF4, RNF51, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1000 Western Blot: 1:100~500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BMI1 Mab is generated from mouse immunized with BMI1 recombinant protein.
Form
Supplied as a liquid, 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Ismail, I.H., et al. J. Cell Biol. 191(1):45-60(2010)|Yang, M.H., et al. Nat. Cell Biol. 12(10):982-992(2010)|Kikuchi, J., et al. Cancer 116(12):3015-3024(2010)|Honig, A., et al. Anticancer Res. 30(5):1559-1564(2010)|Venkataraman, S., et al. PLoS ONE 5 (6), E10748 (2010)USBio References
No references available