Technical Data

030929
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
688CT33.1.3
Grade
Ascites
Applications
E WB
Crossreactivity
Hu
Accession #
NP_005219.2
Gene ID
1956
Gene #
EGFR
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-EGFR, CT (EGFR, ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1)
EGFR, ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100~200
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
EGFR antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1163-1191 amino acids from the C-terminal region of human EGFR.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Ascites
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Abdallah, R.T., et al. J. Biol. Chem. 285(45):35206-35215(2010)|Lu, C., et al. Mol. Cell. Biol. 30(22):5432-5443(2010)|Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010)|Hata, A., et al. J Thorac Oncol 5(10):1524-1528(2010)|Aguirre, A., et al. Nature 467(7313):323-327(2010)
USBio References
No references available
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