Technical Data

030930
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
688CT33.1.3
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_005219.2
Gene ID
1956
Gene #
EGFR
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-EGFR, CT (EGFR, ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1)
EGFR, ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Applications
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000-1:8000 Immunohistochemistry (FFPE): 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1163-1191 in the C-terminal region of human EGFR.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human EGFR.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Abdallah, R.T., et al. J. Biol. Chem. 285(45):35206-35215(2010) 2. Lu, C., et al. Mol. Cell. Biol. 30(22):5432-5443(2010) 3. Rosell, R., et al. Ann. N. Y. Acad. Sci. 1210, 45-52 (2010) 4. Hata, A., et al. J Thorac Oncol 5(10):1524-1528(2010) 5. Aguirre, A., et al. Nature 467(7313):323-327(2010)
USBio References
No references available
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