Technical Data

030934
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
355CT12.6.5
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_001408.2
Gene ID
1975
Gene #
EIF4B
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-EIF4B (EIF4B, Eukaryotic translation initiation factor 4B)
EIF4B, Eukaryotic translation initiation factor 4B

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1000 Western Blot: 1:100~250
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
EIF4B Mab is generated from mouse immunized with EIF4B recombinant protein.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Mokas, S., et al. Mol. Biol. Cell 20(11):2673-2683(2009)|Rozovsky, N., et al. RNA 14(10):2136-2148(2008)|Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Olsen, J.V., et al. Cell 127(3):635-648(2006)
USBio References
No references available
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