Technical Data

030956
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
497CT15.2.5
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_004095.4
Gene ID
2194
Gene #
FASN
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-FASN, ID (FASN, FAS, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase)
FASN, FAS, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
FASN Mab is generated from mice immunized with a KLH conjugated synthetic peptide selected from the center region of human FASN.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)|Nguyen, P.L., et al. J. Clin. Oncol. 28(25):3958-3964(2010)|Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)|Tischler, V., et al. Histopathology 56(6):811-815(2010)|Dorn, C., et al. Int J Clin Exp Pathol 3(5):505-514(2010)
USBio References
No references available
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