Technical Data

031005
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG1
Clone Number
9AT106
Grade
Affinity Purified
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Glutathione S-Transferase (GST)

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Optimal dilution to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant GST fusion protein.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes Glutathione S-Transferase (GST).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31. 2. Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221. 3. Toye, B. et al., (1990) Infect. Immun. 58, 3909. 4. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262¬?267. 5. i, X. et al., (1992) Biochemistry 31, 10169. 6. Davis, A.H. et al. (1993) Biotechnology 11,933-11,936. 7. Maru, Y. et al., (1996) J. Biol. Chem. 271, 15353. 8. Yu, J. et al. (1998) Mol. Cell. Biol. 18, 1379-1387.
USBio References
No references available
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