Technical Data

031072
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b,k
Clone Number
56CT5.1.6
Grade
Affinity Purified
Applications
E FC IF WB
Crossreactivity
Hu Mo
Gene ID
9314
Gene #
KLF4
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-KLF4 (Kruppel-like Factor 4, Epithelial Zinc Finger Protein, EZF, Gut-enriched Krueppel-like Factor, GKLF)

KLF4, which is also known as the gut-enriched Kruppel-like factor (GKLF), is a zinc finger-containing transcription factor that belongs to the Kruppel-like family of transcription factors. KLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. KLF4 is also involved in cell cycle control since expression of KLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.

Applications
Suitable for use in Western Blot, Immunofluorescence, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:4000 Indirect Flow Cytometry: 1:10-1:50 Immunoflourescence: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human KLF4.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human KLF4. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.MCC-555-induced NAG-1 expression is mediated in part by KLF4. Cekanova M, et al. Eur J Pharmacol, 2010 Jul 10. PMID 20385121. 2. Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression. Yori JL, et al. J Biol Chem, 2010 May 28. PMID 20356845. 3. MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. Tian Y, et al. J Biol Chem, 2010 Mar 12. PMID 20075075. 4. Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma. Nakahara Y, et al. Neoplasia, 2010 Jan. PMID 20072650. Kruppel-like factor 4 (Klf4) prevents embryonic stem (ES) cell differentiation by regulating Nanog gene expression. Zhang P, et al. J Biol Chem, 2010 Mar 19. PMID 20071344.
USBio References
No references available
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