031203
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
33AT1045Grade
Affinity PurifiedApplications
ChIP WB KOCrossreactivity
Hu MoGene ID
7799Gene #
PRDM2Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-PRDM2 (RIZ1) (PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger protein RIZ) [KO Validated]
PRDM2, KMT8, RIZ, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger protein RIZ
This tumor suppressor gene is a member of a nuclear histone/protein methyltransferase superfamily. It encodes a zinc finger protein that can bind to retinoblastoma protein, estrogen receptor, and the TPA-responsive element (MTE) of the heme-oxygenase-1 gene. Although the functions of this protein have not been fully characterized, it may (1) play a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma, (2) act as a transcriptional activator of the heme-oxygenase-1 gene, and (3) be a specific effector of estrogen action. Multiple transcript variants encoding different isoforms have been found for this gene.
Applications
Suitable for use in Western Blot and ChIP. Other applications not tested.
Recommended Dilution
Western Blot: 1:1,000 ChIP: 1:100 Optimal dilution to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein G affinity chromatography
Specificity
Recognizes human and mouse PRDM2 (RIZ1). This antibody is specific RIZ1; it does not recognize the R1Z2 isoform.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Genotypes and haplotypes of the estrogen receptor genes, but not the retinoblastoma-interacting zinc finger protein 1 gene, are associated with osteoporosis. Harsl√ûf T, et al. Calcif Tissue Int, 2010 Jul. PMID 20508921.|DNA methylation of the RIZ1 tumor suppressor gene plays an important role in the tumorigenesis of cervical cancer. Cheng HY, et al. Eur J Med Res, 2010 Jan 29. PMID 20159667.|Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer. de Vogel S, et al. Cancer Epidemiol Biomarkers Prev, 2009 Nov. PMID 19843671.|Expression of RIZ1 protein (Retinoblastoma-interacting zinc-finger protein 1) in prostate cancer epithelial cells changes with cancer grade progression and is modulated in vitro by DHT and E2. Rossi V, et al. J Cell Physiol, 2009 Dec. PMID 19746436.|RIZ1 is potential CML tumor suppressor that is down-regulated during disease progression. Lakshmikuttyamma A, et al. J Hematol Oncol, 2009 Jul 14. PMID 19602237.USBio References
No references available