031255-PE
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
PEIsotype
IgG1Clone Number
57CT23.3.4Grade
Affinity PurifiedApplications
FLISA IF IHC WBCrossreactivity
HuGene ID
6657Gene #
SOX2Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
Mouse Anti-SOX2 (SOX2, Transcription factor SOX-2) (PE)
SOX2, Transcription factor SOX-2
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry (Not Tested) and FLISA. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human SOX2.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SOX2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Zhang D, et al. BMC Med Genet, 2010 Jul 28. PMID 20667095. 2. Sholl LM, et al. Am J Surg Pathol, 2010 Aug. PMID 20631605. 3. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983. 4. Gonzalez-Rodriguez J, et al. Br J Ophthalmol, 2010 Aug. PMID 20494911. 4. Reis LM, et al. Mol Vis, 2010 Apr 28. PMID 20454695. References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]. References for MCF7 cell line:1. Soule, HD; et al, (1973). Journal of the National Cancer Institute 51 (5): 1409-1416. [PMID 4357757]. 2 Levenson, AS; Jordan VC. (1997). Cancer Research 57 (15): 3071-3078. [PMID 9242427]. 3. Lacroix, M; Leclercq G. (2004). Breast Research and Treatment 83(3): 249-289.[PMID 14758095].USBio References
No references available