031335
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgM,kClone Number
280CT3.4.6Grade
PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_003371.2Gene ID
7431Gene #
VIMShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-VIME (VIM, Vimentin)
VIM, Vimentin
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1000 Western Blot: 1:100~250
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
VIME Mab is generated from mouse immunized with VIME recombinant protein.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Kers, J., et al. Transplantation 90(5):502-509(2010)|Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010)|Korita, P.V., et al. Anticancer Res. 30(6):2279-2285(2010)|Martins-de-Souza, D., et al. J Psychiatr Res (2010) In press|Li, M., et al. J. Exp. Clin. Cancer Res. 29, 109 (2010)USBio References
No references available