031340
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
510CT8.1.2Grade
AscitesApplications
E WBCrossreactivity
HuAccession #
NP_001070.2Gene ID
7535Gene #
ZAP70Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-ZAP70 (ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70kD zeta-chain associated protein, Syk-related tyrosine kinase)
ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70kD zeta-chain associated protein, Syk-related tyrosine kinase
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Applications
Suitable for use in Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100~500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified His-tagged ZAP70 protein (fragment).
Form
Supplied as a liquid, 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010)|Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010)|Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010)|Kotaskova, J., et al. J Mol Diagn 12(3):328-334(2010)|Trojani, A., et al. Cancer Biomark 6(1):1-9(2010)USBio References
No references available