Mouse Anti-Neurofilament H (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, NEFH, NFH)
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kD, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. 031395 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with 031395 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH.
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:1000 Western Blot: 1:10,000 using chemiluminescence. Immunohistochemistry: 1:4000 in frozen sections in tissue culture and in formalin fixed sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Native NF-H purified from bovine spinal cord
Form
Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some crossreactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. Recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections. Species Crossreactivity: human, rat, mouse, porcine and equine
