Technical Data

031451
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo
Gene ID
84618
Gene #
NT5C1A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-5NT1A, NT (NT5C1A, Cytosolic 5'-nucleotidase 1A, Cytosolic 5'-nucleotidase IA)
NT5C1A, Cytosolic 5'-nucleotidase 1A, Cytosolic 5'-nucleotidase IA

Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates. Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 91-119 in the N-terminal region of human 5NT1A, UniProt Accession #Q9BX13 Cellular Location: Cytoplasm.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human 5NT1A. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Landers, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(22):9004-9009(2009). 2. Lechward, K., et al. Acta Biochim. Biophys. Sin. (Shanghai) 41(4):280-284(2009). 3. Fyrberg, A., et al. Nucleosides Nucleotides Nucleic Acids 25(9-11), 1201-1204 (2006).
USBio References
No references available
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