Technical Data

031509
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_631905.1;
Gene ID
137735
Gene #
ABRA
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ABRA, CT (ABRA, Actin-binding Rho-activating protein, Striated muscle activator of Rho-dependent signaling)
ABRA, Actin-binding Rho-activating protein, Striated muscle activator of Rho-dependent signaling

ABRA acts as an activator of serum response factor (SRF)-dependent transcription possibly by inducing nuclear translocation of MKL1 or MKL2 and through a mechanism requiring Rho-actin signaling (By similarity).

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ABRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-312 amino acids from the C-terminal region of human ABRA.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Lamon, S., et al. J. Physiol. (Lond.) 587 (PT 8), 1795-1803 (2009) : Kuwahara, K., et al. J. Clin. Invest. 117(5):1324-1334(2007) Kuwahara, K., et al. Mol. Cell. Biol. 25(8):3173-3181(2005) Arai, A., et al. J. Biol. Chem. 277(27):24453-24459(2002) Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
USBio References
No references available
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