Technical Data

031562
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Gene ID
116285
Gene #
ACSM1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ACSM1, NT (ACSM1, BUCS1, LAE, MACS1, Acyl-coenzyme A synthetase ACSM1, mitochondrial, Acyl-CoA synthetase medium-chain family member 1, Butyrate-CoA ligase 1, Butyryl-coenzyme A synthetase 1, Lipoate-activating enzyme, Middle-chain acyl-CoA synthetase 1)
ACSM1, BUCS1, LAE, MACS1, Acyl-coenzyme A synthetase ACSM1, mitochondrial, Acyl-CoA synthetase medium-chain family member 1, Butyrate--CoA ligase 1, Butyryl-coenzyme A synthetase 1, Lipoate-activating enzyme, Middle-chain acyl-CoA synthetase 1

ACSM1 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy-and 2,3-or 3,4-unsaturated acids (in vitro). This protein functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ACSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27~57 amino acids from the N-terminal region of human ACSM1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Celis,J.E., et.al., Mol. Cell Proteomics 7 (10), 1795-1809 (2008)|Sullivan,P.F., et.al., Mol. Psychiatry 13 (6), 570-584 (2008)|Haketa,A., et.al., J. Hypertens. 22 (10), 1903-1907 (2004)
USBio References
No references available
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