Technical Data

031565
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_061147.1
Gene ID
55902
Gene #
ACSS2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ACSS2, ID (ACSS2, ACAS2, Acetyl-coenzyme A synthetase, cytoplasmic, Acetate-CoA ligase, Acetyl-CoA synthetase, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme)
ACSS2, ACAS2, Acetyl-coenzyme A synthetase, cytoplasmic, Acetate--CoA ligase, Acetyl-CoA synthetase, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme

This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ACSS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-290 amino acids from the Central region of human ACSS2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)|Yilmaz, S., et al. Genomics 96(1):57-65(2010)|Ban, H.J., et al. BMC Genet. 11, 26 (2010) :|Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)|Yun, M., et al. J. Nucl. Med. 50(8):1222-1228(2009)
USBio References
No references available
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