Rabbit Anti-ACTL6B, NT (ACTL6B, ACTL6, BAF53B, Actin-like protein 6B, 53kD BRG1-associated factor B, Actin-related protein Baf53b, ArpNalpha, BRG1-associated factor 53B) (Biotin)
ACTL6B, ACTL6, BAF53B, Actin-like protein 6B, 53kD BRG1-associated factor B, Actin-related protein Baf53b, ArpNalpha, BRG1-associated factor 53B
ACTL6B is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested) and ELISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 42-70 in the N-terminal region of human ACTL6B.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ACTL6B.
