Rabbit Anti-ADM, ID (ADM, AM, Adrenomedullin, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide)
ADM, AM, Adrenomedullin, Proadrenomedullin N-20 terminal peptide, ProAM N-terminal 20 peptide
ADM, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes.
Applications
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000-1:2000 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 69-96 of human ADM. Species Sequence Homology: bovine
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ADM.
