Technical Data

031671
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E
Crossreactivity
Hu
Gene ID
4299
Gene #
AFF1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-AF4, ID (MLLT2, AF-4 protein, Proto-oncogene AF4, FEL protein)
MLLT2, AF-4 protein, Proto-oncogene AF4, FEL protein

MLLT2 is involved in acute leukemias through a chromosomal translocation t(4;11)(q21;q23) that involves mllt2 and mll/hrx. AF-4 (MLLT2), AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains. AF4 is a serine- and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus. The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant negative mechanism in leukemogenesis.

Applications
Suitable for use in ELISA
Recommended Dilution
ELISA: 1:1,000
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
AF4 (MLLT2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human MLLT2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Bursen, A., et al., Oncogene 23(37):6237-6249 (2004).|Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).|Caslini, C., et al., Leukemia 18(6):1064-1071 (2004).|Bertrand, F.E., et al., Leukemia 17(12):2454-2459 (2003).|Reichel, M., et al., Leukemia 15(2):286-288 (2001).
USBio References
No references available
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