Technical Data

031750
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Accession #
NP_001014431.1; NP_001014432.1
Gene ID
207
Gene #
AKT1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-AKT1 (Thr308) (AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha)
AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from human AKT1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Zhao, W.D., et al. Infect. Immun. 78(11):4809-4816(2010)|Sanematsu, F., et al. Circ. Res. 107(9):1102-1105(2010)|Nicodemus, K.K., et al. Arch. Gen. Psychiatry 67(10):991-1001(2010)|Treekitkarnmongkol, W., et al. World J. Gastroenterol. 16(32):4047-4054(2010)|Chen, J., et al. PLoS ONE 5 (8), E12293 (2010) :
USBio References
No references available
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