031825-APC
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
APCIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IF IHC WBCrossreactivity
HuAccession #
NP_976316.1Gene ID
23600Gene #
AMACRShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeRabbit Anti-AMACR, CT (AMACR, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase) (APC)
AMACR, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase
This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry (Not Tested) and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 323-351 in the C-terminal region of human AMACR.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human AMACR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010) 2. Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010) 3. Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010) 4. Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009) 5. Mubiru, J.N., et al. Gene 327(1):89-98(2004)USBio References
No references available