Technical Data

031825
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC IF IHC WB
Crossreactivity
Hu
Accession #
NP_976316.1
Gene ID
23600
Gene #
AMACR
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-AMACR, CT (AMACR, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase)
AMACR, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 323-351 in the C-terminal region of human AMACR.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human AMACR.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010) 2. Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010) 3. Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010) 4. Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009) 5. Mubiru, J.N., et al. Gene 327(1):89-98(2004)
USBio References
No references available
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