031959
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
NP_001124500.1;Gene ID
83734Gene #
ATG10Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-APG10L, CT (S116) (ATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10)
ATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the C-terminal region of human APG10L.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)|Shao, Y., et al. Autophagy 3(1):10-16(2007)|Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :|Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)USBio References
No references available