Technical Data

032047
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_055599.1; O15085
Gene ID
9826
Gene #
ARHGEF11
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ARHGEF11, ID (ARHGEF11, KIAA0380, Rho guanine nucleotide exchange factor 11, PDZ-RhoGEF)
ARHGEF11, KIAA0380, Rho guanine nucleotide exchange factor 11, PDZ-RhoGEF

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ARHGEF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-632 amino acids from the Central region of human ARHGEF11.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Akiyama, K., et al. J. Hum. Genet. (2010) In press :|Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Ying, Z., et al. Arterioscler. Thromb. Vasc. Biol. 29(10):1657-1663(2009)|Banerjee, J., et al. Biochemistry 48(33):8032-8043(2009)|Zheng, M., et al. BMC Struct. Biol. 9, 36 (2009) :
USBio References
No references available
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