Technical Data

032176
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Gene ID
55054
Gene #
ATG16L1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1)
ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84~114 amino acids surrounding amino acid L92 of human APG16L.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) |Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) |Greenberg JT. Dev Cell. 8(6):799-801. (2005) |Levine B. Cell. 120(2):159-62. (2005) |Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
USBio References
No references available
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