Technical Data

032232
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Gene ID
9474
Gene #
ATG5
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)
ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:500. Heat induced antigen retrieval with EDTA Buffer, pH 9.0 is recommended. Immunofluorescence: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human ATG5. Species Sequence Homology: zebrafish, bovine, mouse, porcine and rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human ATG5

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). 2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). 3. Greenberg JT. Dev Cell. 8(6):799-801. (2005). 4. Levine B. Cell. 120(2):159-62. (2005). 5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). 6. Hammond E.M., et al. FEBS Lett. 425:391-395(1998). 7. Strausberg R.L., et al. PNAS 99:16899-16903(2002). 8. Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995). 9. Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998). 10. Mizushima N., et al. J. Cell Biol. 152:657-668(2001)
USBio References
No references available
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