Technical Data

032246
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Gene ID
79065
Gene #
ATG9A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ATG9A, CT (ATG9A, APG9L1, Autophagy-related protein 9A, APG9-like 1, mATG9)
ATG9A, APG9L1, Autophagy-related protein 9A, APG9-like 1, mATG9

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 724~754 amino acids from the C-term of human APG9L1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) |Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) |Greenberg JT. Dev Cell. 8(6):799-801. (2005) |Levine B. Cell. 120(2):159-62. (2005) |Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
USBio References
No references available
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