Technical Data

032268
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_689509.1
Gene ID
478
Gene #
ATP1A3
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ATP1A3, ID (ATP1A3, Sodium/potassium-transporting ATPase subunit alpha-3, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3)
ATP1A3, Sodium/potassium-transporting ATPase subunit alpha-3, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 805-833 in the central region of human ATP1A3. Species Sequence Homology: chicken, mouse and rat.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human ATP1A3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Einholm, A.P., et al. J. Biol. Chem. 285(34):26245-26254(2010). 2. Floyd, R.V., et al. Reprod Sci 17(4):366-376(2010). 3. Hauck, C., et al. Eur. J. Pharmacol. 622 (1-3), 7-14 (2009). 4. Blanco-Arias, P., et al. Hum. Mol. Genet. 18(13):2370-2377(2009). 5. Goldstein, I., et al. Biol. Psychiatry 65(11):985-991(2009)
USBio References
No references available
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