Technical Data

032283
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_001002027.1; NP_005166.1
Gene ID
516
Gene #
ATP5G1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ATP5G1, ID (ATP5G1, ATP synthase lipid-binding protein, mitochondrial, ATP synthase proteolipid P1, ATPase protein 9, ATPase subunit c)
ATP5G1, ATP synthase lipid-binding protein, mitochondrial, ATP synthase proteolipid P1, ATPase protein 9, ATPase subunit c

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ATP5G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from the Central region of human ATP5G1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human, mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Vives-Bauza, C., et al. Mol. Biol. Cell 21(1):131-139(2010)|Wang, H.L., et al. Cytogenet. Genome Res. 109 (4), 533 (2005) :|Cross, R.L. Nature 427(6973):407-408(2004)|Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)|Wang, H., et al. Nature 396(6708):279-282(1998)
USBio References
No references available
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