Technical Data

032299
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_653184.2
Gene ID
245973
Gene #
ATP6V1C2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ATP6V1C2, ID (ATP6V1C2, V-type proton ATPase subunit C 2, Vacuolar proton pump subunit C 2)
ATP6V1C2, V-type proton ATPase subunit C 2, Vacuolar proton pump subunit C 2

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ATP6V1C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human ATP6V1C2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Gruber, G. Biochem. Soc. Trans. 33 (PT 4), 883-885 (2005) :|Morel, N. Biol. Cell 95(7):453-457(2003)|Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)|Kawasaki-Nishi, S., et al. FEBS Lett. 545(1):76-85(2003)
USBio References
No references available
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