032342
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E FC IHC WBCrossreactivity
HuAccession #
NP_004646.3Gene ID
8313Gene #
AXIN2Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-AXIN2, CT (AXIN2, Axin-2, Axin-like protein, Axis inhibition protein 2, Conductin)
AXIN2, Axin-2, Axin-like protein, Axis inhibition protein 2, Conductin
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: 1:50-1:100 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 816-843 in the C-terminal region of human AXIN2, UniProt Accession #Q9Y2T1. Species sequence homology: mouse, rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human AXIN2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Inkster, B., et al. Neuroimage (2010) In press, 2. Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010), 3. Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010), 4. Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870 (2009), 5. Olschwang, S., et al. J Oncol 2009, 306786 (2009), |5. Dong, X., et al. Cytogenet. Cell Genet. 93 (1-2),:26-28 (2001), 7. Liu, W., et al. Nat. Genet. 26(2):146-147(2000), 8. von Kries, J.P., et al. Nat. Struct. Biol. 7(9):800-807(2000), 9. Kikuchi, A. Cytokine Growth Factor Rev. 10 (3-4):255-265 (1999), 10. Mai, M., et al. Genomics 55(3):341-344(1999)USBio References
No references available