Rabbit Anti-B4GALT2, CT (B4GALT2, Beta-1,4-galactosyltransferase 2, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase)
B4GALT2, Beta-1,4-galactosyltransferase 2, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2, Lactose synthase A protein, N-acetyllactosamine synthase, Nal synthase, Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase, Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
B4GALT2 is one of type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
B4GALT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297~326 amino acids from the C-terminal region of human B4GALT2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
