Technical Data

032414
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
DB E
Crossreactivity
Hu
Accession #
NP_004313.1
Gene ID
572
Gene #
BAD
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-BAD, phosphorylated (Y76) (BAD, BBC6, BCL2L8, Bcl2 antagonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter)
BAD, BBC6, BCL2L8, Bcl2 antagonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq].

Applications
Suitable for use in Dot Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Dot blot 1:500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Phospho-BAD-Y76 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y76 of human BAD.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Chen, B., et al. Am. J. Physiol., Cell Physiol. 299 (5), C968-C976 (2010) :|Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)|Galmiche, A., et al. Mol. Cancer Res. 8(8):1116-1125(2010)|Cerioni, L., et al. Methods Mol. Biol. 648, 291-301 (2010) :|Yu, B., et al. J. Exp. Clin. Cancer Res. 29, 107 (2010) :
USBio References
No references available
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