Technical Data

032449
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001158245.1; O15382; NP_001181.2
Gene ID
587
Gene #
BCAT2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-BCAT2, CT (BCAT2, BCATM, BCT2, ECA40, Branched-chain-amino-acid aminotransferase, mitochondrial, Placental protein 18)
BCAT2, BCATM, BCT2, ECA40, Branched-chain-amino-acid aminotransferase, mitochondrial, Placental protein 18

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BCAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human BCAT2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Coles, S.J., et al. Biochemistry 48(3):645-656(2009)|Yennawar, N.H., et al. J. Biol. Chem. 281(51):39660-39671(2006)|Goto, M., et al. J. Biol. Chem. 280(44):37246-37256(2005)|Conway, M.E., et al. Biochemistry 43(23):7356-7364(2004)|Yennawar, N., et al. Acta Crystallogr. D Biol. Crystallogr. 57 (PT 4), 506-515 (2001) :
USBio References
No references available
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